Identification and recombinant expression of anandamide hydrolyzing enzyme from Dictyostelium discoideum
نویسندگان
چکیده
منابع مشابه
Lysosomal enzyme secretory mutants of Dictyostelium discoideum.
Dictyostelium discoideum secretes a number of lysosomal enzymes during axenic growth and upon suspension in a low ionic strength, non-nutrient buffer (standard secretion conditions). These secretory characteristics have allowed us to identify 74 lysosomal enzyme secretory mutants generated by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The majority of these mutants fell into one of four c...
متن کاملExpression of growth factors in Dictyostelium discoideum.
Growth factors and their binding proteins are important proteins regulating mammalian cell proliferation and differentiation so there is considerable interest in producing them as recombinant proteins, especially in hosts that do not already produce a complex mixture of growth factors. Many growth factors require post-translational modifications making them unsuitable for production in Escheric...
متن کاملDictyostelium discoideum
Dictyostelium discoideum is a cellular slime mold [4] that serves as an important model organism [5] in a variety of fields. Cellular slime molds have an unusual life cycle. They exist as separate amoebae, but after consuming all the bacteria in their area they proceed to stream together to form a multicellular organism. These features make them a valuable tool for studying developmental proces...
متن کاملIdentification and characterization of a Dictyostelium discoideum ribosomal protein gene.
We have identified a developmentally repressed large-subunit ribosomal protein gene of Dictyostelium discoideum based on sequence similarity to other ribosomal proteins. Protein rpl7 is homologous to large subunit ribosomal proteins from the rat and possibly to Mycoplasma capricolum and Escherichia coli, but is not similar to three sequenced ribosomal proteins in Dictyostelium. The rpl7 gene is...
متن کاملCharacterization of RapGAP1 from Dictyostelium discoideum
Diese Dissertation wurde selbständig und ohne unerlaubte Hilfsmittel angefertigt.
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2012
ISSN: 1471-2180
DOI: 10.1186/1471-2180-12-124